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Cygnus Protein A Mix-N-Go? ELISA: The Gold Standard for Protein A Residue Detection


Cygnus Protein A Mix-N-Go? Series ELISA Kits

Cygnus Protein A Mix-N-Go? Series ELISA Kits are specifically designed for detecting residues of most natural and recombinant Protein A. They adopt a revolutionary one-step sample processing procedure. Compared with traditional Protein A ELISA methods, the Mix-N-Go? Series ELISA Kits simplify experimental procedures, shorten assay time, and simultaneously improve method reliability.

 

Advantages of Protein A Mix-N-Go? Kits:

· No boiling or centrifugation steps required — reducing the use and calibration of equipment.

· Robust method — ensuring irreversible dissociation of Protein A from IgG.

· Rapid access to test results — assay time is 2 hours shorter than that of other kits.

· Higher precision — fewer steps lead to less error.

· All reagents required for detection and pre-prepared standards are provided in the kit.

 

Comparison of Operational Procedures: Cygnus Protein A Mix-N-Go? vs. Protein A Detection Kits of Other Brands

 

Feature

Cygnus Protein A Mix-N-Go? ELISA Kit

Brand A

Brand B

Sample Handling

 

 

 

Protein A-IgG irreversible dissociation?

Yes

No

No

Boiling required?

No

Yes

Yes

Centrifugation required?

No

Yes

Yes

Allowable IgG concentration in sample

≤20 mg/mL

1-15 mg/mL

Must dilute to 50 μg/mL

Total sample processing time

10 min

45 min

54 min

 

Step

Cygnus Protein A Mix-N-Go? ELISA Kit

Brand A

Brand B

Reagent Preparation

All reagents are ready-to-use

Require preparation to working concentration

Require preparation to working concentration

Reagent Prep Time

None

~10 min (diluting standards)

~10 min (diluting standards)

Reaction Method

One-step reaction

Multi-step reaction

Multi-step reaction

Wash Steps

1

4

3

Total Hands-on Time

1h 45min

2h 5min

3h 54min

 

One-Step Protein A Mix-N-Go? Sample Processing Procedure


Three-Step Routine Protein A Sample Processing Procedure


 

Guide for Selecting Protein A Mix-N-Go? ELISA Kits

Cygnus Protein A Mix-N-Go ELISA Kits have been widely used in batch release testing for numerous commercial therapeutic monoclonal antibodies. They have also undergone specific testing and validation for compatibility with most commercial Protein A chromatography resins. To target Protein A ligands from different brand sources, Cygnus has designed and developed a variety of detection kits; the recommended item numbers are shown in the table below.

Protein A Resin

Resin Manufacture

Recommended Cygnus Protein A

Mix-N-Go? Kit Item No.

Correction Factor*

Amsphere? A3

JSR Life Sciences®

F740

Specialized Kit

Amsphere? A+

JSR Life Sciences®

F1075 New Launched

Specialized Kit

Eshmuno® A

MilliporeSigma®/

MerckMillipore?

F610

1

J.T.Baker® BAKERBOND®

PROchievA? Protein A

Avantor®

F965

Specialized Kit

KanCapA? 3G Protein A

Kaneka®

F950

Specialized Kit

MabSelect?

Cytiva®

F600

1

MabSelect? SuRe,

MabSelect? SuRe XL

Cytiva®

F610

1

MabSelect? PrismA?

Cytiva®

F610

1.6

Praesto? Jetted A50

Purolite®

F610

1.3

TOYOPEARL® SuperA (R50)
TOYOPEARL® AF-rProtein A HC-650F (R40)
TOYOPEARL® AF-rProtein A-650F (R28)

Tosoh Bioscience®

F910

Specialized Kit

*The Correction Factor accounts for calibration discrepancies caused by differences in the molecular weights of Protein A ligands. It must be applied to the Protein A concentration values calculated from the F610 standard curve to determine the residual Protein A concentration corresponding to the relevant resin.

 

Protein A Mix-N-Go? ELISA Kit Ordering Information

Product Name

Item Number

Protein A Mix-N-Go? ELISA Kit

F600

Protein A Mix-N-Go? ELISA Kit for UnNatural Protein A Constructs

F610

Protein A Mix-N-Go? ELISA for Amsphere? A3 Ligand

F740

Protein A Mix-N-Go? ELISA for Amsphere? A+ Ligand   New Launched

F1075

Tosoh R50, R40 and R28 Protein A Mix-N-Go? ELISA

F910

KANEKA KanCapA? 3G Protein A Mix-N-Go? ELISA

F950

J.T.Baker® BAKERBOND® PROchievA? Protein A Mix-N-Go? ELISA

F965

 

Cygnus Technologies, LLC. provides products and analytical methods for the biotechnology and biopharmaceutical industries, aiming to accelerate the R&D phase and enhance product quality. The bioprocess residual detection kits developed and manufactured by Cygnus are used to detect specific impurities from over 50 different expression systems. As an expert in high-sensitivity analytical technologies focused on immunoassays for biotechnological applications, Cygnus’ products and services have been utilized by nearly all major biopharmaceutical companies for more than 25 years.



As the exclusive distributor of Cygnus in China, Beijing XMJ Technology Co., Ltd. has established long-term and stable cooperative relationships with numerous well-known domestic pharmaceutical enterprises and CRO/CMO companies. Over the years, XMJ's products and services have helped many enterprises accelerate the R&D phase, improve drug quality, purity and safety, optimize R&D processes more efficiently, shorten time-to-market for products, and reduce QC costs. If you are interested in the aforementioned products, please feel free to send email to info@xmjsci.com or visit the website www.gq44.cn for more information.